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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NSF
All Species:
23.33
Human Site:
S81
Identified Species:
36.67
UniProt:
P46459
Number Species:
14
Phosphosite Substitution
Charge Score:
-0.36
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
P46459
NP_006169.2
744
82594
S81
I
G
Q
E
I
E
V
S
L
Y
T
F
D
K
A
Chimpanzee
Pan troglodytes
XP_511626
842
92301
S154
I
G
Q
E
I
E
V
S
L
Y
T
F
D
K
A
Rhesus Macaque
Macaca mulatta
XP_001105450
854
93325
S191
I
G
Q
E
I
E
V
S
L
Y
T
F
D
K
A
Dog
Lupus familis
XP_548044
752
83541
S81
I
G
Q
E
I
E
V
S
L
Y
T
F
D
K
A
Cat
Felis silvestris
Mouse
Mus musculus
P46460
744
82595
A81
I
G
Q
D
I
E
V
A
L
Y
S
F
D
K
A
Rat
Rattus norvegicus
Q9QUL6
744
82634
A81
I
G
Q
D
I
E
V
A
L
Y
S
F
D
K
A
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
Chicken
Gallus gallus
XP_418094
740
82158
S81
I
G
Q
E
I
D
V
S
L
Y
T
F
D
K
S
Frog
Xenopus laevis
P23787
805
89193
K81
D
D
T
C
S
D
E
K
I
R
M
N
R
V
V
Zebra Danio
Brachydanio rerio
Q7ZU99
806
89405
K81
D
D
T
C
S
D
E
K
V
R
M
N
R
V
V
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
P46461
745
82537
R81
I
N
Q
E
L
E
V
R
P
Y
R
F
D
A
S
Honey Bee
Apis mellifera
XP_001120201
743
82591
R82
L
N
Q
E
I
E
V
R
P
Y
H
F
N
P
T
Nematode Worm
Caenorhab. elegans
Q94392
824
91316
T145
L
D
Q
E
V
R
V
T
P
F
T
F
Q
Q
S
Sea Urchin
Strong. purpuratus
NP_999752
746
82671
A83
I
D
Q
P
I
Q
A
A
P
Y
R
F
D
P
S
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Q9M0Y8
742
81469
S90
T
G
D
M
V
S
V
S
R
F
V
P
P
E
N
Baker's Yeast
Sacchar. cerevisiae
P18759
758
84038
K100
L
N
Q
D
V
Q
A
K
A
F
D
L
F
K
Y
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
88
86.8
98.1
N.A.
98.1
97.8
N.A.
N.A.
93.6
26.2
25
N.A.
62.4
65.8
50.6
64.8
Protein Similarity:
100
88.2
87.1
98.8
N.A.
99.7
99.7
N.A.
N.A.
96.5
44.2
44.4
N.A.
77.7
81.1
66.2
80.5
P-Site Identity:
100
100
100
100
N.A.
80
80
N.A.
N.A.
86.6
0
0
N.A.
53.3
46.6
33.3
40
P-Site Similarity:
100
100
100
100
N.A.
100
100
N.A.
N.A.
100
13.3
13.3
N.A.
66.6
60
73.3
60
Percent
Protein Identity:
N.A.
N.A.
N.A.
43.5
44.9
N.A.
Protein Similarity:
N.A.
N.A.
N.A.
64.6
64.2
N.A.
P-Site Identity:
N.A.
N.A.
N.A.
20
13.3
N.A.
P-Site Similarity:
N.A.
N.A.
N.A.
40
46.6
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
0
0
0
0
0
14
20
7
0
0
0
0
7
40
% A
% Cys:
0
0
0
14
0
0
0
0
0
0
0
0
0
0
0
% C
% Asp:
14
27
7
20
0
20
0
0
0
0
7
0
60
0
0
% D
% Glu:
0
0
0
54
0
54
14
0
0
0
0
0
0
7
0
% E
% Phe:
0
0
0
0
0
0
0
0
0
20
0
74
7
0
0
% F
% Gly:
0
54
0
0
0
0
0
0
0
0
0
0
0
0
0
% G
% His:
0
0
0
0
0
0
0
0
0
0
7
0
0
0
0
% H
% Ile:
60
0
0
0
60
0
0
0
7
0
0
0
0
0
0
% I
% Lys:
0
0
0
0
0
0
0
20
0
0
0
0
0
54
0
% K
% Leu:
20
0
0
0
7
0
0
0
47
0
0
7
0
0
0
% L
% Met:
0
0
0
7
0
0
0
0
0
0
14
0
0
0
0
% M
% Asn:
0
20
0
0
0
0
0
0
0
0
0
14
7
0
7
% N
% Pro:
0
0
0
7
0
0
0
0
27
0
0
7
7
14
0
% P
% Gln:
0
0
80
0
0
14
0
0
0
0
0
0
7
7
0
% Q
% Arg:
0
0
0
0
0
7
0
14
7
14
14
0
14
0
0
% R
% Ser:
0
0
0
0
14
7
0
40
0
0
14
0
0
0
27
% S
% Thr:
7
0
14
0
0
0
0
7
0
0
40
0
0
0
7
% T
% Val:
0
0
0
0
20
0
74
0
7
0
7
0
0
14
14
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
0
0
0
0
0
0
0
0
0
67
0
0
0
0
7
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _